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snrna seq data  (Broad Clinical Labs)
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Broad Clinical Labs snrna seq data
<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Snrna Seq Data, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclei rna sequencing snrna seq data  (Broad Clinical Labs)
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<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Nuclei Rna Sequencing Snrna Seq Data, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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data new snrna sequencing  (Qiagen)
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<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Data New Snrna Sequencing, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleus rna sequencing snrna seq datasets  (Broad Clinical Labs)
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Broad Clinical Labs nucleus rna sequencing snrna seq datasets
<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Nucleus Rna Sequencing Snrna Seq Datasets, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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data mouse snrna sequencing data geo gse286360  (R&D Systems)
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<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Data Mouse Snrna Sequencing Data Geo Gse286360, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna libraries for 10x genomics snrna-sequencing  (10X Genomics)
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10X Genomics cdna libraries for 10x genomics snrna-sequencing
<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Cdna Libraries For 10x Genomics Snrna Sequencing, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human colon enteric neuron single nuclei rna sequencing snrna seq  (Broad Clinical Labs)
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Broad Clinical Labs human colon enteric neuron single nuclei rna sequencing snrna seq
<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Human Colon Enteric Neuron Single Nuclei Rna Sequencing Snrna Seq, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snrna library preparation and sequencing libraries  (10X Genomics)
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<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Snrna Library Preparation And Sequencing Libraries, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single-nucleus rna sequencing (snrna-seq)  (Novogene)
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Novogene single-nucleus rna sequencing (snrna-seq)
<t>snRNA‐Seq</t> <t>identifies</t> distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.
Single Nucleus Rna Sequencing (Snrna Seq), supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


snRNA‐Seq identifies distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.

Journal: Journal of Neurochemistry

Article Title: A High‐Resolution Transcriptomic Atlas of Cell Types in the Ventral Visual Thalamus

doi: 10.1111/jnc.70406

Figure Lengend Snippet: snRNA‐Seq identifies distinct neuronal and glial cell types in vLGN. (A) Schematic of viral infection following AAV1‐Cre injection. (B) Trans‐synaptic labeling of retinorecipient cells in ventral lateral geniculate nucleus (vLGN) of H2B‐transgenic mice following intraocular delivery of AAV1‐Cre. White dashed line outlines vLGN. mCherry(H2B)‐labeled cells are present in vLGN (arrowheads). (C) Identification and isolation of H2b‐mCherry+ nuclei by FACS sorting. (D) UMAP (uniform manifold approximation and projection) plot of 10 266 cell nuclei from the ventral lateral geniculate nucleus (vLGN), clustered by expression of high‐variance genes and colored according to cluster identity. (E) Violin plots showing expression of pan‐neuronal marker genes ( Syn1, Rbfox3 ); oligodendrocyte marker gene ( Olig1 ); macrophage marker gene ( Cx3cr1 ); endothelial cell marker gene ( Cldn5 ); pericyte marker gene ( Vtn ); astrocyte marker genes ( Aldoc ); microglia marker gene ( Mrc1 ); excitatory neuron marker gene ( Slc17a6, Slc17a7 ); and inhibitory neuron marker genes ( Gad1, Gad2 ). (F) UMAP recolored to illustrate expression levels of marker genes. (G) UMAP clustered by expression of high‐variance genes and colored according to predicted cell type.

Article Snippet: Resource availability : A user‐friendly interface for visualizing and exploring the snRNA‐Seq data is available at the Broad Institute Single Cell Portal at https://singlecell.broadinstitute.org/single_cell/study/SCP3575 .

Techniques: Infection, Injection, Labeling, Transgenic Assay, Isolation, Expressing, Marker

snRNA‐Seq elucidates 13 retinorecipient neuronal types in vLGN. (A) UMAP (uniform manifold approximation and projection) of 4489 neuron cell nuclei from the ventral lateral geniculate nucleus, clustered by expression of high‐variance genes and colored according to cluster identity. (B) Neuronal UMAP recolored to illustrate expression of 89 mCherry+ vLGN nuclei following AAV injection in neurons. (C) Dot plot of neuronal clusters showing the expression of inhibitory marker genes ( Gad1 and Gad2 ) and excitatory marker genes ( Slc17a6 and Slc17a7 ) separated by original identity. (D) Dot plot of neuronal clusters showing the expression of Nxph1 (vLGNe) separated by original identity. (E) Neuron UMAP recolored to illustrate expression levels of Nxph1 .

Journal: Journal of Neurochemistry

Article Title: A High‐Resolution Transcriptomic Atlas of Cell Types in the Ventral Visual Thalamus

doi: 10.1111/jnc.70406

Figure Lengend Snippet: snRNA‐Seq elucidates 13 retinorecipient neuronal types in vLGN. (A) UMAP (uniform manifold approximation and projection) of 4489 neuron cell nuclei from the ventral lateral geniculate nucleus, clustered by expression of high‐variance genes and colored according to cluster identity. (B) Neuronal UMAP recolored to illustrate expression of 89 mCherry+ vLGN nuclei following AAV injection in neurons. (C) Dot plot of neuronal clusters showing the expression of inhibitory marker genes ( Gad1 and Gad2 ) and excitatory marker genes ( Slc17a6 and Slc17a7 ) separated by original identity. (D) Dot plot of neuronal clusters showing the expression of Nxph1 (vLGNe) separated by original identity. (E) Neuron UMAP recolored to illustrate expression levels of Nxph1 .

Article Snippet: Resource availability : A user‐friendly interface for visualizing and exploring the snRNA‐Seq data is available at the Broad Institute Single Cell Portal at https://singlecell.broadinstitute.org/single_cell/study/SCP3575 .

Techniques: Expressing, Injection, Marker